New models of archaeal ammonia oxidation indicate a key role for NO produced by nitrite reductase encoded by the gene AnirK22 providing a clear link to the production of N2O, possibly via hybrid N2O formation22,23,24,25. A role for AOA mediated N2O production has been suggested in the oceans, with process, natural abundance isotope values and molecular data supporting this idea12,20,26,27. There has, however, been no formal experimental characterization of N2O production at oxygen concentrations representative of the margins of an OMZ (2l1) where N2O accumulates2,28 and/or the abundance of AOA (or any other candidate organisms) in representative samples of the ocean13..
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